Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX116172 diluted at 1:1000

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NCKIPSD antibody [C1C3] (GTX116172) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.