HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with NDUFS1 antibody (GTX113787) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDUFS1 antibody (GTX113787) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using NDUFS1(GTX113787) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS1(GTX113787) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min