Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NDUFS3 antibody detects NDUFS3 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: NDUFS3 stained by NDUFS3 antibody (GTX105835) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
NDUFS3 antibody (GTX105835) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NDUFS3 antibody (GTX105835) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: NDUFS3 protein stained by NDUFS3 antibody (GTX105835) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NDUFS3 stained by NDUFS3 antibody (GTX105835) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min