Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: THP-1
B: HL-60
12% SDS PAGE
GTX113504 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NDUFS3(GTX113504) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX113504) diluted by 1:1000.