Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody [1B6] (GTX70281) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Detection of human NEK2 protein using GeneTex NEK2 1B6 monoclonal antibody (GTX70281) in Jurkat whole cell extract.