Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (GTX636768) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (GTX636768) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
NEK7 stained by NEK7 antibody [HL1348] (GTX636768) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min