NF-M antibody detects NF-M protein at cytoskeleton by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NF-M protein stained by NF-M antibody (GTX85461) diluted at 1:250.
Red: F-Actin , stained by F-Actin antibody [NH3] (GTX20205) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Dissociated cell culture prepared from an adult rat brain. NFM (green staining) stains neurites in a punctate fashion, corresponding to neuritic varicosities. As a control, rabbit GFAP antibodies were used to co-stain astrocytes, producing a red fluorescence. Blue nuclei demonstrate DAPI DNA staining

ICC/IF analysis of mouse cortical neurons using GTX85461 NF-M antibody.
Green : Primary antibody
Red : GFAP
Blue : DAPI
Dilution : 1:2000

The data was published in the journal PLoS Biol in 2015.PMID: 26406915