Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with NFAT4 antibody (GTX133744) diluted at 1:500 and competitor's antibody (sc-8321) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFAT4 antibody (GTX133744) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.