HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFIA antibody (GTX114580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NFIA antibody detects NFIA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NFIA antibody detects NFIA protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFIA protein stained by NFIA antibody (GTX114580) diluted at 1:1000.
Blue: Hoechst 33342 staining.

NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
NFIA antibody (GTX114580) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min