Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIC antibody (GTX114583) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC(GTX114583) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min