HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NFYB antibody detects NFYB protein at nucleus in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 μg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 μg of NFYB antibody (GTX121922) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (GTX121922) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFYB protein stained by NFYB antibody (GTX121922) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NFYB antibody detects NFYB protein at nucleus in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NFYB antibody (GTX121922) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis (whole mount) of zebrafish embryo, using NFYB antibody (GTX121922) at 1:200 dilution.

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (GTX121922) at 1:300 dilution.