Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody [GT1185] (GTX00947) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

WB analysis of wild-type (WT) and NFkB p65 knockout (KO) HeLa cell extracts using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Laoding : 25μg

WB analysis of various samples using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:1000
Loading : 25μg

IP analysis of HeLa cell lysate using GTX00947 NFkB p65 antibody [GT1185].
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

IHC-P analysis of rat spleen tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100

IHC-P analysis of human lung cancer tissue section using GTX00947 NFkB p65 antibody [GT1185].
Dilution : 1:100