Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody [N3C3] (GTX107910) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
NLK antibody
GTX107910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX107910 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(GTX107910) antibody at 1:100 dilution.

NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (GTX107910) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min