Western Blot: NMDA NR2B Phosphospecific Antibody [GTX31085] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.