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NMDAR2B (phospho Tyr1472) antibody

Orthogonal
Anti-NMDAR2B (phospho Tyr1472) antibody used in Western Blot (WB). GTX31086

Cat. No. GTX31086

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Primate
Package
50 μl ($519)
See all NMDAR2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

166 kDa. ( Note )

Product Note

Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either gamma-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr1472 of NMDA NR2B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate ionotropic receptor NMDA type subunit 2B , GluN2B

Cellular Localization

Cell membrane

Background

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Database

Research Area

DATA IMAGES

Anti-NMDAR2B (phospho Tyr1472) antibody used in Western Blot (WB). GTX31086

GTX31086 WB Image

Orthogonal

WB analysis of rat hippocampal lysate using GTX31086 NMDAR2B (phospho Tyr1472) antibody.
Lane 1 : without lambda phosphatase
Lane 2 : lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 519