APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100 - 1:2000 |
1:50 - 1:500 |
1:10 - 1:500 |
Assay dependent |
1:10 - 1:500 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nucleotide binding oligomerization domain containing 2 , ACUG , BLAU , BLAUS , CARD15 , CD , CLR16.3 , IBD1 , NLRC2 , NOD2B , PSORAS1 , YAOS
Cellular Localization
Cytoplasm,Membrane,Mitochondrion
Background
This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX30615 FACS Image
FACS (Intracellular staining) analysis of Raji cells using GTX30615 NOD2 antibody [2D9]. Blue : Primary antibody Orange : isotype control Dilution : 5 μg/ml Fixation : 4% PFA Permibilization : 0.1% saponin
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GTX30615 FACS Image
FACS (Intracellular staining) analysis of THP-1 cells using GTX30615 NOD2 antibody [2D9]. Blue : Primary antibody Orange : isotype control Dilution : 5 μg/ml Fixation : 4% PFA Permibilization : 0.1% saponin
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GTX30615 WB Image
WB analysis of THP-1, RAW264.7, and U2OS cell lysate using GTX30615 NOD2 antibody [2D9]. Dilution : 2 μg/ml
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GTX30615 WB Image
WB analysis of THP-1 cell lysate using GTX30615 NOD2 antibody [2D9]. Dilution : 2 μg/ml
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GTX30615 WB Image
WB analysis of HT-29 cell lysate using GTX30615 NOD2 antibody [2D9]. Loading : 50μg
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GTX30615 ICC/IF Image
ICC/IF analysis of A431 cells using GTX30615 NOD2 antibody [2D9]. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:200 Fixation : 10% formalin Permibilization : 0.5% Triton-X100
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GTX30615 WB Image
WB analysis of HEK293 overexpressing NOD2 cell lysate using GTX30615 NOD2 antibody [2D9].
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REFERENCE
There are currently no references for NOD2 antibody [2D9] (GTX30615). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for NOD2 antibody [2D9] (GTX30615). Be the first to share your experience with this product.