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NOTCH1 antibody [3E12]

KO/KD Overexpression
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in Western Blot (WB). GTX84019
Anti-NOTCH1 antibody [3E12] used in Western Blot (WB). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019
Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019
Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019

Cat. No. GTX84019

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E12

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($539)
See all NOTCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

273 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

notch receptor 1 , AOS5 , AOVD1 , TAN1 , hN1

Cellular Localization

Cell membrane

Background

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.

Database

Research Area

DATA IMAGES

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of breast tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in Western Blot (WB). GTX84019

GTX84019 WB Image

KO/KD

WB analysis of wild type and NOTCH1 knock out HeLa cell lysate(10μg per lane) using GTX84019 NOTCH1 antibody [3E12].
Dilution : 1:500

Anti-NOTCH1 antibody [3E12] used in Western Blot (WB). GTX84019

GTX84019 WB Image

Overexpression

WB analysis of HEK293T cells transfected with NOTCH1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84019 NOTCH1 antibody [3E12].
Loading : 5 ug per lane

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of kidney tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of thyroid tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019

GTX84019 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84019 NOTCH1 antibody [3E12].

Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019

GTX84019 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with NOTCH1 plasmid using GTX84019 NOTCH1 antibody [3E12].

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of lung tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of colon tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in IHC (Paraffin sections) (IHC-P). GTX84019

GTX84019 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84019 NOTCH1 antibody [3E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-NOTCH1 antibody [3E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84019

GTX84019 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84019 NOTCH1 antibody [3E12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539