Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NOTCH1 antibody [HL1281] (GTX636683) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NOTCH1 antibody [HL1281] (GTX636683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.