Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NPEPPS antibody [N3C2-2], Internal detects NPEPPS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NPEPPS stained by NPEPPS antibody [N3C2-2], Internal (GTX115247) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.