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NQO1 antibody [A180]

Orthogonal
Anti-NQO1 antibody [A180] used in IHC (Paraffin sections) (IHC-P). GTX30626
Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626
Anti-NQO1 antibody [A180] used in Flow cytometry (FACS). GTX30626
Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626
Anti-NQO1 antibody [A180] used in IHC (Paraffin sections) (IHC-P). GTX30626
Anti-NQO1 antibody [A180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30626
Anti-NQO1 antibody [A180] used in Flow cytometry (FACS). GTX30626
Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626
Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626
Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

Cat. No. GTX30626

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A180

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat, Dog, Primate
Package
100 μl ($419)
See all NQO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50
IHC-P 1:100
IHC-Fr 1:100
FACS 1 - 2 μg/mL
IP 1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Does not cross-react with NQO2.

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 150mM NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant NQO1 from human lung [UniProt# P15559]

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_625991

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NAD(P)H quinone dehydrogenase 1 , DHQU , DIA4 , DTD , NMOR1 , NMORI , QR1

Cellular Localization

Cytoplasm

Background

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NQO1 antibody [A180] used in IHC (Paraffin sections) (IHC-P). GTX30626

GTX30626 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30626 NQO1 antibody [A180].

Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

GTX30626 WB Image

WB analysis of A431 cell lysate using GTX30626 NQO1 antibody [A180].

Anti-NQO1 antibody [A180] used in Flow cytometry (FACS). GTX30626

GTX30626 FACS Image

FACS (Intracellular staining) analysis of U87-MG cells using GTX30626 NQO1 antibody [A180].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

GTX30626 WB Image

WB analysis of A549 cell lysate using GTX30626 NQO1 antibody [A180].
Lane 1 : 0.5 ug/ml
Lane 2 : 2 ug/ml
Dilution : 2 μg/ml

Anti-NQO1 antibody [A180] used in IHC (Paraffin sections) (IHC-P). GTX30626

GTX30626 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30626 NQO1 antibody [A180].

Anti-NQO1 antibody [A180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30626

GTX30626 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX30626 NQO1 antibody [A180].
Green : primary antibody
Red : Beta-tubulin
Blue : DAPI

Anti-NQO1 antibody [A180] used in Flow cytometry (FACS). GTX30626

GTX30626 FACS Image

FACS (Intracellular staining) analysis of A549 cells using GTX30626 NQO1 antibody [A180].
Filled histogram : primary antibody
Open histogram : isotype control

Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

GTX30626 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

GTX30626 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-NQO1 antibody [A180] used in Western Blot (WB). GTX30626

GTX30626 WB Image

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

REFERENCE

Application Reference

Bai X et al. Oxid Med Cell Longev 2019; 2019 : 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Jeongsu Han et al. Oxidative Medicine and Cellular Longevity 2019; 2019 : Article ID 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419