HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody (GTX103179) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Molt-4 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NRF1 antibody (GTX103179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NRF1 antibody detects NRF1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
NRF1 antibody (GTX103179) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NRF1 antibody detects NRF1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
NRF1 antibody (GTX103179) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NRF1 antibody detects NRF1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NRF1 stained by NRF1 antibody (GTX103179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NRF1 antibody detects NRF1 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
NRF1 antibody (GTX103179) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

NRF1 antibody detects NRF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF1 stained by NRF1 antibody (GTX103179) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

NRF1 antibody immunoprecipitates NRF1 protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of NRF1 protein by 4 μg NRF1 antibody (GTX103179)
10 % SDS-PAGE
The immunoprecipitated NRF1 protein was detected by NRF1 antibody (GTX103179) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

The data was published in the journal Nat Commun in 2019. PMID: 30833558