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NRF2 (phospho Ser40) antibody

Anti-NRF2 (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX02873
Anti-NRF2 (phospho Ser40) antibody used in ELISA (ELISA). GTX02873
Anti-NRF2 (phospho Ser40) antibody used in Western Blot (WB). GTX02873
Anti-NRF2 (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX02873
Anti-NRF2 (phospho Ser40) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02873

Cat. No. GTX02873

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all NRF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human nrf2 around the phosphorylation site of Ser40.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm, cytosol , Nucleus

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-NRF2 (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX02873

GTX02873 IHC-P Image

IHC-P analysis of human normal tissues adjacent to mammary cancer using GTX02873 NRF2 (phospho Ser40) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-NRF2 (phospho Ser40) antibody used in ELISA (ELISA). GTX02873

GTX02873 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02873 NRF2 (phospho Ser40) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-NRF2 (phospho Ser40) antibody used in Western Blot (WB). GTX02873

GTX02873 WB Image

WB analysis of HT-29 cell lysate using GTX02873 NRF2 (phospho Ser40) antibody.

Anti-NRF2 (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX02873

GTX02873 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX02873 NRF2 (phospho Ser40) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-NRF2 (phospho Ser40) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02873

GTX02873 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells (4h of LPS treatment) using GTX02873 NRF2 (phospho Ser40) antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

REFERENCE

Application Reference

Yammine A et al. Curr Res Toxicol 2024; 6 : 100153 Cytoprotective effects of α-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, oleic acid and α-tocopherol on 7-ketocholesterol - Induced oxiapoptophagy: Major roles of PI3-K / PDK-1 / Akt signaling pathway and glutathione peroxidase activity in cell rescue.
A Yammine et al. CRTOX 2024; 6 : 100153 Cytoprotective effects of α-linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, oleic acid and α-tocopherol on 7-ketocholesterol – Induced oxiapoptophagy: Major roles of PI3-K / PDK-1 / Akt signaling pathway and glutathione peroxidase activity in cell rescue
Xu Z et al. Turk J Med Sci 2023; 53 (2) : 446-454 Resveratrol attenuated high intensity exercise training-induced inflammation and ferroptosis via Nrf2/FTH1/GPX4 pathway in intestine of mice.
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Package List Price ($)
$ 399