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NT5C2 antibody [N2C1], Internal

Anti-NT5C2 antibody [N2C1], Internal used in Western Blot (WB). GTX105719
Anti-NT5C2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105719
Anti-NT5C2 antibody [N2C1], Internal used in Western Blot (WB). GTX105719

Cat. No. GTX105719

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NT5C2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Mouse liver , H1299 , HeLaS3

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NT5C2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951032

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5'-nucleotidase, cytosolic II , GMP , NT5B , PNT5 , SPG45 , SPG65 , cN-II

Cellular Localization

Cytoplasm

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NT5C2 antibody [N2C1], Internal used in Western Blot (WB). GTX105719

GTX105719 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX105719 diluted at 1:1000

Anti-NT5C2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105719

GTX105719 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NT5C2(GTX105719) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NT5C2 antibody [N2C1], Internal used in Western Blot (WB). GTX105719

GTX105719 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX105719 diluted at 1:500

REFERENCE

Application Reference

Chen YT et al. Sci Rep 2020; 10 (1) : 16087 NT5C2 methylation regulatory interplay between DNMT1 and insulin receptor in type 2 diabetes.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169