Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 μg of NUF2 antibody [GT312] (GTX632247) .
Western blot analysis was performed using NUF2 antibody [GT312] (GTX632247) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody [GT312] (GTX632247) diluted at 1:1000.

NUF2 antibody [GT312] detects NUF2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NUF2 stained by NUF2 antibody [GT312] (GTX632247) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min