Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NUP50(GTX106428) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis.
A. 50 μg mouse testis lysate/extract
10 % SDS-PAGE
NUP50 antibody [C3], C-term (GTX106428) dilution: 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (GTX106428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human normal colon, using NUP50(GTX106428) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min