NUP98 antibody [2H10]

Cat. No. GTX00697
Cat. No.  GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
GTX00697 ICC/IF Image

ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

1 / 9
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
GTX00697 WB Image

WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml

2 / 9
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
GTX00697 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

3 / 9
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
GTX00697 ICC/IF Image

ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min

4 / 9
Anti-NUP98 antibody [2H10] used in  (). GTX00697
GTX00697 Image

Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.

5 / 9
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
GTX00697 ICC/IF Image

ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.

6 / 9
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
GTX00697 WB Image

WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml

7 / 9
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
GTX00697 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min

8 / 9
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
GTX00697 WB Image

WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000

9 / 9
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in  (). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
Anti-NUP98 antibody [2H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00697
Anti-NUP98 antibody [2H10] used in Western Blot (WB). GTX00697
  • Host
    Rat
  • Clonality
    Monoclonal
  • Clone Name
    2H10
  • Isotype
    IgG2c
  • Applications
    WB ICC/IF Dot ELISA
  • Reactivity
    Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe
See all NUP98 products