Wild-type (WT) and NEK1 knockout (KO) HeLa cell extracts (100 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.

Nek1 antibody detects Nek1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nek1 protein stained by Nek1 antibody (GTX130828) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nek1 antibody (GTX130828) diluted at 1:1000.