Nestin antibody [GT623]

Cat. No. GTX630201
Cat. No.  GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
GTX630201 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
GTX630201 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT623
  • Isotype
    IgG1
  • Applications
    WB ICC/IF
  • Reactivity
    Human
Summary
Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.
See all Nestin products