Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Netrin 1 antibody (GTX121508) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed MCF-7, using Netrin G1 ligand(GTX121508) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Netrin G1 ligand(GTX121508) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX121508 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.