Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Nkx2.5 antibody (GTX133155) diluted at 1:500 and competitor's antibody (sc-14033) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Nkx2.5 antibody detects Nkx2.5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Green: Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:250.
Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Nkx2.5 antibody (GTX133155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Nkx2.5 antibody detects Nkx2.5 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Nkx2.5 stained by Nkx2.5 antibody (GTX133155) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min