Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Nucleobindin 1 antibody [HL2532] detects secreted Nucleobindin 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Nucleobindin 1 antibody [HL2532] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nucleobindin 1 stained by Nucleobindin 1 antibody [HL2532] (GTX638901) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).