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OAT antibody [AT23A2]

Anti-OAT antibody [AT23A2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50004
Anti-OAT antibody [AT23A2] used in Western Blot (WB). GTX50004

Cat. No. GTX50004

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT23A2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all OAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human OAT (33-439aa) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ornithine aminotransferase , GACR , HOGA , OATASE , OKT

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-OAT antibody [AT23A2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50004

GTX50004 ICC/IF Image

Immunofluorescence of human HeLa cells stained with monoclonal anti-human OAT anitbody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Anti-OAT antibody [AT23A2] used in Western Blot (WB). GTX50004

GTX50004 WB Image

Tissue lysates of mouse liver (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399
Currently in production