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OneStep Polymer HRP anti-rabbit (Ready-to-Use)

Comparison
OneStep Polymer HRP anti-rabbit (Ready-to-Use). GTX83399
OneStep Polymer HRP anti-rabbit (Ready-to-Use). GTX83399

Cat. No. GTX83399

Reference 8

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Rabbit
Package
100 test ($179)
See all Polymer HRP anti-Rabbit products
PRODUCT

Summary

Fully optimized for IHC and can be used with an autostainer or manual immunohistochemistry staining methods.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

Based on IEP, no cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.

PROPERTIES

Form

Liquid

Buffer

TBS, 1% BSA, 10% heat-inactivated animal Serum

Preservative

0.1% ProClin 150

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Purified Rabbit IgG, whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography

Purity

> 95% by SDS-PAGE

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_11164964

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

anti-rabbit HRP polymer , IHC Polymer , antirabbit HRP polymer , anti rabbit HRP polymer

Background

Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.

GeneTex’s One-Step anti-rabbit IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This reagent has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.

Features and Benefits
‧ Lower background: Amplification without biotin avoids background from endogenous biotin
‧ Higher sensitivity than standard IHC detection
‧ Faster: Staining time reduced to 15 minutes!
‧ Compatible with either manual or automated systems
‧ Better results on difficult-to-work antibodies and nuclear staining
‧ Save cost due to further dilution of primary antibody

DATA IMAGES

OneStep Polymer HRP anti-rabbit (Ready-to-Use). GTX83399

GTX83399 IHC-P Image

Comparison

PTBP1 antibody detects PTBP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PTBP1 stained by PTBP1 antibody (GTX101481) diluted at 1:500.
The signal was developed by OneStep Polymer HRP anti-rabbit (Ready-to-Use) (left), VECTASTAINR ABC-HRP Kits (middle), and conventional secondary antibody (right).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

OneStep Polymer HRP anti-rabbit (Ready-to-Use). GTX83399

GTX83399 IHC-P Image

Triple Positive Breast Cancer sections were stained with rabbit anti-CD3 antibody and then detected by GTX83399 OneStep Polymer HRP anti-rabbit (Ready-to-Use).

REFERENCE

Application Reference

Mansur A et al. Biomedicines 2023; 11 (8) Combination Therapy with Trastuzumab and Niraparib: Quantifying Early Proliferative Alterations in HER2+ Breast Cancer Models.
Ameer Mansur et al. Biomedicines 2023; 11 (8) : 2090 Combination Therapy with Trastuzumab and Niraparib: Quantifying Early Proliferative Alterations in HER2+ Breast Cancer Models
Yun Lu et al. Molecules 2021; 26 (6)
Amemori S et al. Eur J Neurosci 2020; 51 (3) : 731-741 Microstimulation of primate neocortex targeting striosomes induces negative decision-making.
Si Y et al. J Steroid Biochem Mol Biol 2020; 200 : 105650 The vitamin D activator CYP27B1 is upregulated in muscle fibers in denervating disease and can track progression in amyotrophic lateral sclerosis.
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Package List Price ($)
$ 179