Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Optineurin(GTX105447) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Optineurin antibody immunoprecipitates Optineurin protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Optineurin protein by 4 μg Optineurin antibody (GTX105447)
7.5 % SDS-PAGE
The immunoprecipitated Optineurin protein was detected by Optineurin antibody (GTX105447) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Optineurin(GTX105447) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (GTX105447) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.