WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) cell lysates using GTX54848 Orexin Receptor 2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

ICC/IF analysis of PFA-fixed human colon cancer cells using GTX54848 Orexin Receptor 2 antibody.
Panel A : Primary antibody
Panel B : Live view of the same field as in (A)
Panel C : DNA dye Hoechst 33342
Dilution : 1:500

IHC-Fr analysis of rat brain tissue using GTX54848 Orexin Receptor 2 antibody. Hoechst 33342 is used as  the counterstain (blue).
Panel A :  In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML).
Panel B :  In the parietal cortex, staining is evident in neural cell bodies.
Panel C :  In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. 
Dilution : 1:500

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates using GTX54848 Orexin Receptor 2 antibody preincubated with or without immunogen peptide.
Dilution : 1:400