P2X5 antibody [HL2773] detects P2X5 protein by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2X5 stained by P2X5 antibody [HL2773] (GTX639638) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled Raji whole cell extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X5 antibody [HL2773] (GTX639638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X5 antibody [HL2773] (GTX639638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.