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P2Y2 antibody [HL2801]

Recombinant antibody Overexpression
Anti-P2Y2 antibody [HL2801] used in IHC (Paraffin sections) (IHC-P). GTX639689
Anti-P2Y2 antibody [HL2801] used in IHC (Paraffin sections) (IHC-P). GTX639689
Anti-P2Y2 antibody [HL2801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639689
Anti-P2Y2 antibody [HL2801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639689

Cat. No. GTX639689

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2801

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all P2Y2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

This antibody was raised against mouse P2Y2 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain mouse of P2Y2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2Y, G-protein coupled 2 , P2U1 , P2Y2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Predicted to enable A1 adenosine receptor binding activity; ATP binding activity; and G protein-coupled UTP receptor activity. Acts upstream of or within positive regulation of mucus secretion. Predicted to be located in apical plasma membrane and basolateral plasma membrane. Predicted to colocalize with early endosome; lysosome; and mitochondrion. Orthologous to human P2RY2 (purinergic receptor P2Y2). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-P2Y2 antibody [HL2801] used in IHC (Paraffin sections) (IHC-P). GTX639689

GTX639689 IHC-P Image

P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (GTX639689) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2Y2 antibody [HL2801] used in IHC (Paraffin sections) (IHC-P). GTX639689

GTX639689 IHC-P Image

P2Y2 antibody [HL2801] detects P2Y2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (GTX639689) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-P2Y2 antibody [HL2801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639689

GTX639689 ICC/IF Image

P2Y2 antibody [HL2801] detects P2Y2 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (GTX639689) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-P2Y2 antibody [HL2801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639689

GTX639689 ICC/IF Image

Overexpression

P2Y2 antibody [HL2801] detects P2Y2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: P2Y2 stained by P2Y2 antibody [HL2801] (GTX639689) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations