Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] (GTX106794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PACAP antibody [N1C3] detects PACAP protein at cytosol on human normal colon mucosa with lymphocyte by immunohistochemical analysis.
Sample: Paraffin-embedded normal colon mucosa with lymphocyte.
PACAP antibody [N1C3] (GTX106794) dilution: 1:500.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PACAP antibody [N1C3] (GTX106794) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PACAP antibody [N1C3] detects PACAP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample:Frozen section of mouse brain.
PACAP antibody [N1C3] (GTX106794) diluted at 1:500.

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunofluorescent analysis.
Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PACAP antibody [N1C3] detects PACAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PACAP stained by PACAP antibody [N1C3] (GTX106794) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min