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PAD4 antibody

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531
Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531
Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531
Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04531
Anti-PAD4 antibody used in Western Blot (WB). GTX04531

Cat. No. GTX04531

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PADI4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PADI4(Accession Q9UM07), corresponding to amino acid residues M1-A33.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidyl arginine deiminase 4 , PAD , PAD4 , PADI5 , PDI4 , PDI5

Cellular Localization

Cytoplasm , Nucleus , Cytoplasmic granule

Background

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531

GTX04531 IHC-P Image

IHC-P analysis of rat lung tissue using GTX04531 PAD4 antibody.
Antigen retrieval : Heat mediated sodium citrate buffer
Dilution : 1:100

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531

GTX04531 IHC-P Image

IHC-P analysis of human ovarian cancer using GTX04531 PAD4 antibody.
Antigen retrieval : Heat mediated sodium citrate buffer
Dilution : 1:100

Anti-PAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04531

GTX04531 IHC-P Image

IHC-P analysis of human prostate cancer using GTX04531 PAD4 antibody.
Antigen retrieval : Heat mediated sodium citrate buffer
Dilution : 1:100

Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04531

GTX04531 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX04531 PAD4 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-PAD4 antibody used in Western Blot (WB). GTX04531

GTX04531 WB Image

WB analysis of mouse heart tissue lysate and HepG2 whole cell lysate using GTX04531 PAD4 antibody.
The lane on the right was HepG2 whole cell lysate treated with blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399