Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAICS antibody (GTX118340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PAICS(GTX118340) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
E: HL-60
F: NCI-H929
10% SDS PAGE
GTX118340 diluted at 1:1000