Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000 and competitor's antibody (#9542) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PARP1 antibody [N1N2], N-term(GTX112839) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.

PARP antibody [N1N2], N-term detects PARP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP protein stained by PARP antibody [N1N2], N-term (GTX112839) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N1N2], N-term (GTX112839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PARP1 antibody [N1N2], N-term immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N1N2], N-term (GTX112839)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N1N2], N-term (GTX112839) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]