U87-MG whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PAX3 antibody [HL160] detects PAX3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
Green: PAX3 stained by PAX3 antibody [HL160] (GTX635463) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] (GTX635463) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.