Name: PAX8 antibody
Brand: GeneTex
SKU: GTX101583
Price: 419.00 USD

GTX101583 ChIP assay Image

PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody (GTX101583) The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

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GTX101583 IP Image

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody (GTX101583) 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody (GTX101583) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

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GTX101583 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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GTX101583 WB Image

293T whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:1000.

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GTX101583 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8(GTX101583) antibody at 1:200 dilution.

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GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX101583 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
PAX8 antibody (GTX101583) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleolus by immunohistochemical analysis.
Sample: Paraffin-embedded DLD1 xenograft .
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
PAX8 stained by PAX8 antibody (GTX101583) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX101583 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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