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PBK antibody [N2C3]

KO/KD
Anti-PBK antibody [N2C3] used in Immunoprecipitation (IP). GTX113982
Anti-PBK antibody [N2C3] used in Western Blot (WB). GTX113982
Anti-PBK antibody [N2C3] used in Western Blot (WB). GTX113982

Cat. No. GTX113982

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PBK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , HeLa , 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619532

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PDZ binding kinase , CT84 , HEL164 , Nori-3 , SPK , TOPK

Background

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PBK antibody [N2C3] used in Immunoprecipitation (IP). GTX113982

GTX113982 IP Image

Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 μg of PBK antibody [N2C3] (GTX113982).
Western blot analysis was performed using PBK antibody [N2C3] (GTX113982).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PBK antibody [N2C3] used in Western Blot (WB). GTX113982

GTX113982 WB Image

PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody (GTX113982) diluted at a dilution of 1:1000.

Anti-PBK antibody [N2C3] used in Western Blot (WB). GTX113982

GTX113982 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody [N2C3] (GTX113982) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169