HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.

IP analysis of Jurkat cell lysate using GTX12188 PCAF antibody.
Lane 1 : 5 μg/mL antibody
Lane 2 : Negative control: without antibody
Lane 3 : 10 μg/mL antibody
Lane 4 : Negative control: without cell extract

WB analysis of human PCAF recombinant protein using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Negative control (without antibody)

WB analysis of (Lane 1-2) HeLa, (Lane 3-4) HepG2, (Lane 5-6) Jurkat cell lysate using GTX12188 PCAF antibody at 2 μg/mL.
Lane 1,3,5 : Antibody 2 μg/mL
Lane 2,4,6 : Antibody 2 μg/mL + PCAF immunizing peptide

WB analysis of Y79 cell lysate using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)