Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PCPE-1(GTX104154) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PCOLCE antibody [N1C3] (GTX104154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.