Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL2293] (GTX638348) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PD-L1 antibody [HL2293] detects PD-L1 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
PD-L1 stained by PD-L1 antibody [HL2293] (GTX638348) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min