APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human PD-L1 Intracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human PD-L1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD274 molecule , B7-H , B7H1 , PD-L1 , PDCD1L1 , PDCD1LG1 , PDL1
Cellular Localization
Cell membrane,Early endosome membrane
Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX640395 WB Image
Wild-type (WT) and PD-L1 knockout (KO) MDA-MB-231 cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL2985] (GTX640395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX640395 ICC/IF Image
PD-L1 antibody [HL2985] detects PD-L1 protein by immunofluorescent analysis.Sample: MDA-MB-231 cells were fixed in ice-cold MeOH for 5 min.Green: PD-L1 stained by PD-L1 antibody [HL2985] (GTX640395) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX640395 WB Image
Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD-L1 antibody [HL2985] (GTX640395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PD-L1 antibody [HL2985] (GTX640395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PD-L1 antibody [HL2985] (GTX640395). Be the first to share your experience with this product.