GeneTex

  • Country / Location Selection

United States (US)

PD1 antibody

Overexpression
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435
Anti-PD1 antibody used in Western Blot (WB). GTX128435
Anti-PD1 antibody used in Western Blot (WB). GTX128435
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435
Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435
Anti-PD1 antibody used in Western Blot (WB). GTX128435

Cat. No. GTX128435

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

NCI-H929 , HL-60 , DDDDK-tagged PD1-transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human PD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885778

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 1 , CD279 , PD-1 , PD1 , SLEB2 , hPD-1 , hPD-l , hSLE1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane of inflammatory cells in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PD1 antibody (GTX128435) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD1 antibody used in Western Blot (WB). GTX128435

GTX128435 WB Image

PD-1 antibody detects PD-1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PD-1 antibody (GTX128435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD1 antibody used in Western Blot (WB). GTX128435

GTX128435 WB Image

PD1 antibody detects PDCD1 protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
PD1 antibody (GTX128435) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128435

GTX128435 IHC-P Image

PD1 antibody detects PD1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
PD1 stained by PD1 antibody (GTX128435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PD1 antibody used in Western Blot (WB). GTX128435

GTX128435 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PD1 antibody (GTX128435) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Shan-Chih Lee et al. Pharmaceuticals 2023; 16 (9) : 1188 Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model
Application : IHC-P
Submit a Reference

REVIEW

There are currently no reviews for PD1 antibody (GTX128435). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169