Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) SH-SY5Y whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PDGF Receptor beta (phospho Tyr751) antibody detects PDGF Receptor beta (phospho Tyr751) protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Mock and treated NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor beta (phospho Tyr751) protein stained by PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.